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These particles contained DNA that was enriched for the terminal sequences of the AAV genomes and was accessible to digestion with micrococcal nuclease. The slower-sedimenting components from the 1.45 band were unstable in CsCl and were present in a greater proportion early after infection. Pulse-chase analysis failed to demonstrate a precursor-product relationship between these two bands. Light is composed of particles called photons. Continuous-label experiments showed that early after infection, both components (1.45 and 1.41) were generated in the same amounts, but this was followed by a relative increase in the proportion of the 1.41 component over the 1.45 particles. According to quantum theory of light, light has dual nature, it acts both as particles as well as wave. High frequency photons carry more energy, sufficient to overcome the binding energy (b) of electrons in the metal, and eject electrons. Electrons are ejected when are hit by high frequency photons. The main component from this band had a smaller S value (109) than the main component from the 1.41 band (111S), although both had the same DNA/protein ratio and the same density in metrizamide gradients. When he explained the photoelectric effect, that light was carried by particles (photons) became clear. The 1.45 band was found to be composed of a heterogeneous group of viral particles that could be subfractionated by velocity sedimentation. KB cells coinfected with adenovirus and adeno-associated virus (AAV) yielded two kinds of infectious AAV particles that banded in CsCl at densities of 1.45 and 1.41 g/cm(2), respectively.